علوم زیستی دریا
Narges Saremi; Saeed Keyvanshokooh; Seyed Mohammad Mousavi; Hamid Mohammadiazarm
Abstract
Abstract
This study was conducted to investigate the effects of nano-selenium (Se) and vitamin C on the growth and nutritional indicators and body composition of common carp (Cyprinus carpio) juveniles. Experimental diets were prepared with three levels of nanoselenium (0, 0.5, and 1 mg/kg) and three ...
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Abstract
This study was conducted to investigate the effects of nano-selenium (Se) and vitamin C on the growth and nutritional indicators and body composition of common carp (Cyprinus carpio) juveniles. Experimental diets were prepared with three levels of nanoselenium (0, 0.5, and 1 mg/kg) and three levels of vitamin C (0, 500 and 1000 mg/kg). The fish were randomly divided into nine experimental groups in triplicate and fed with experimental diets for eight weeks. The results showed that fish fed with C500Se1 and C500Se0.5 diets had a significantly higher specific growth rate than other treatments (P<0.05). The lowest feed conversion ratio was observed in the C1000Se1 treatment and the highest in the control group (P<0.05). Also, based on the results, no significant change was observed in the carcass biochemical composition of common carp juveniles (P>0.05). In general, the results show that the composition of nanoselenium and vitamin C had positive effects on the growth and nutritional indicators of common carp, and it can be stated that the diet enriched with 500 mg of vitamin C and 1 mg of nanoselenium is recommended to increase the growth of common carp.
Introduction
Selenium (Se) is an essential micronutrient that participates in the structure of selenoproteins with vital biological functions in humans and animals. Selenium is involved in antioxidant defense, growth, fertility, immunity, digestion, and absorption of food in farmed animals, and has been used in nutritional studies on various fish species. Combined supplementation of nanoselenium and vitamin C improved growth performance and health status of Nile tilapia (Oreochromis niloticus) (Dawood et al., 2020). However, the positive effects of combining nanoselenium with vitamin C in common carp have not been investigated. As studies on other species suggest that the positive effects of nanoselenium can be enhanced in combination with other micronutrients (Khalil et al., 2022), the present study aimed to investigate the effects of nanoselenium and vitamin C on growth performance and body biochemistry of common carp (Cyprinus carpio) juveniles.
Materials and methods
Experimental diets were prepared with three levels of nanoselenium (0, 0.5 and 1 mg/kg) and three levels of vitamin C (0, 500 and 1000 mg/kg). The fish were randomly divided into 9 experimental groups in triplicates and were fed with experimental diets for 8 weeks. The fish were fed twice a day (9:00 am and 17:00 pm). Each experimental treatment was aerated with constant aeration and about 20% of water was daily exchanged. During the experiment, water quality parameters were daily monitored. Temperature ranges from 27.5 to 28.3oC, pH was between 7.5 and 7.8, dissolved oxygen was 7.2 to 7.5 mg/ml and photoperiods were about 10 hours of light and 14 hours of dark and was adjusted according to the daily natural light. At the end of the experimental period, all fish in each treatment were biometrically measured and the growth parameters were calculated.
Results
The results showed that fish fed with C500Se1 and C500Se0.5 diets had a significantly higher specific growth rate than other treatments (P<0.05). The lowest feed conversion ratio was observed in the C1000Se1 treatment and the highest in the control group (P<0.05). Also, based on the results, no significant change was observed in the carcass biochemical composition of common carp juvenile (P>0.05).
Conclusion
In the present study, the addition of nanoselenium and vitamin C, alone or in combination, to the diet of common carp increased the final weight and SGR and improved the FCR. These findings are in agreement with studies that investigated the effects of Nanoselenium or vitamin C supplements in their separate application on common carp (Ashouri et al., 2015; Ghafarifarsani et al., 2022) and other fish species (Çiçek and Özoğul, 2021, Dawood et al. 2021). In general, the results show that the composition of nanoselenium and vitamin C had positive effects on the growth and nutritional indicators of common carp and it can be stated that the diet enriched with 500 mg of vitamin C and 1 mg of nanoselenium is recommended to increase the growth of common carp.
علوم زیستی دریا
Amin Ghaedtaheri; Saeed Keyvanshokouh; Seyed Mohammad Mousavi; Hossein Pasha-Zanoosi
Abstract
his study aimed to investigate the effect of selenium and magnesium nanoparticles and their composition on growth performance, nutritional indices, survival rate and hepatic accumulation of nano selenium and nano-magnesium in Asian Sea Bass (Lates calcalifer) with mean weight: 32.78 ± 1.16 g for ...
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his study aimed to investigate the effect of selenium and magnesium nanoparticles and their composition on growth performance, nutritional indices, survival rate and hepatic accumulation of nano selenium and nano-magnesium in Asian Sea Bass (Lates calcalifer) with mean weight: 32.78 ± 1.16 g for 42 days. After four weeks of acclimation to the experimental conditions, 96 fish were randomly distributed in 12 fiberglass 300-liter tanks in equal numbers. Four treatments including nano-selenium and nano-magnesium free as control group, 4 mg nano-selenium treatment, 0.5 g nano-magnesium treatment and combination of 4 mg nano-selenium and 0.5 g nano-magnesium were investigated in this study. The fish were fed daily in two times based on station. Fish biometry was performed in all treatments at the end of the experiment. Samples needed to measure liver accumulation were collected at the end of the period. The results of this study showed that the final weight in the treatment of selenium nanoparticles was significantly different (P <0.05), percentage of body weight gain and specific growth rate in selenium + magnesium nanoparticles treatment was significantly different compared to the control group (P <0.05). Nutritional indices of protein yield coefficient, feed intake and feed conversion ratio were not significantly different among experimental treatments (P> 0.05). The results showed that the survival rate was not significantly different among experimental treatments (P> 0.05). The highest levels of selenium and magnesium accumulation in liver were related to selenium nanoparticles and selenium + magnesium nanoparticles, respectively, which were significantly different from other treatments (P <0.05).
علوم زیستی دریا
Hamed Deilamy pour; Seyed Mohammad Mousavi; Mohammad Zakeri; Preeta Kochanian; Saeed Keyvanshokouh
Abstract
The aim of this study was to investigate the effect of selenium, magnesium nanoparticles and their composition on growth and digestive enzymes of Asian seabass with a mean weight of 32.78±1.16 g for 42 days. After adaptation to the experimental conditions, 96 fish were randomly distributed in ...
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The aim of this study was to investigate the effect of selenium, magnesium nanoparticles and their composition on growth and digestive enzymes of Asian seabass with a mean weight of 32.78±1.16 g for 42 days. After adaptation to the experimental conditions, 96 fish were randomly distributed in 12 fiberglass cylinder 300-liter tanks in equal numbers. Four treatments including control treatment, 4mg / kg nanoselenium, 500mg / kg nanomagnasium and combination of 4mg/ kg nanoselenium with 500mg / kg nanomagnasium were investigated. The fish were fed daily in satiation and up to 3% of body weight in two time a day. Samples needed to measure growth indices and digestive enzymes were collected at the end of the period. The results showed that in the fish fed the combined treatment, the highest body weight gain was 131.33±26.58 and the specific growth rate was 2.08±0.28 and there was a significant difference between the control treatment (65.01±21.26 and 1.23±0.32, respectively) (P <0.05). The results of this study showed that digestive enzymes of alkaline phosphatase, lipase, trypsin and chymotrypsin were significantly different between experimental treatments (P <0.05), so that the highest activity of alkaline phosphatase in nanoselenium treatment (611.06±82.00U/mg protein), the highest amount of lipase (0.86±0.03 U/mg protein), trypsin (0.054 ± 0.001 U/mg protein), and chymotrypsin (0.214±0.004U/mg protein) was seen in nanomagnesium treatment (P <0.05), whereas the amylase enzyme showed no significant difference between the experimental treatments (P> 0.05). The results of this study showed that the addition of selenium and magnesium nanoparticles to the diet of Asian Sea bass had positive effects on growth performance and digestive enzymes and suggested that magnesium nanoparticle supplementation (500 mg/kg) could be used alone or in combination with selenium nanoparticles (4 mg/kg nano-selenium and 500 mg / kg nano-magnesium) in the diet of Asian Sea bass.